Phytoquest Compound Libraries

Phytoquest has developed four different libraries with the following characteristics:

Library Name	Description	Details
Phytopure Library	Pure mainly non-polar compounds	~1,000 compounds that are not commercially available and of which approximately 700 are novel
Fraction Library	Less pure non-polar compounds	~3,000 fractions
Diversity Library	Commercially-available compounds	~100 compounds supplied on micro-titre plates for screening purposes
Iminosugar Library	Mainly unavailable elsewhere	~100 compounds and ongoing program of characterization

Careful selectivity inherent in the Phytoquest methods ensures that Phytopure and Fraction libraries display DIVERSITY, NOVELTY and FUNCTION as described below:

Diversity

• Isolated from over 750 plant genera including annuals, perennials, shrubs & trees
• Plants are mainly temperate and obtained from many different habitats including cultivated sites and wild locations and many grown by PhytoQuest
• Several extraction routes utilized resulting in compounds of widely ranging polarity
• A broad range of chemical classes represented
• Ubiquitous plant compounds avoided at compound isolation stage

Novelty

• Abundance of novel compounds is a prerequisite for plant selection
• For each selected plant only those fractions containing novel compounds progress
• Isolation of duplicate and commercially available compounds avoided by 2-stage spectral matching
• PhytoQuest superior techniques and experienced chemists enable the isolation of more unusual compounds
• Structural database matching ensures no duplication within library
• All compounds checked against commercial databases to confirm non-availability from other sources
• Selectivity results in two thirds of Phytopure being novel (as determined by Chapman & Hall Dictionary of Natural Products) with the remaining third not commercially available

Function

• PhytoQuest ensures that compounds with molecular weights outside the pharmaceutical-relevant range are rejected
• A high degree of functionality (displayed by absorbance spectra) is used as a criteria for selecting compounds for isolation
• Chromatographic methods used ensure that logP’s of compounds fall within Lipinski parameters
• Certain chemical groups (eg pigments and fats) are avoided during isolation

Phytoquest management follow a best practice based approach to the management of the Libraries, focused on ensuring provenance and reproducibility:

Provenance

• Identification of all plants material is confirmed by in-house taxonomic experts
• Batches of plant material are logged and tracked

Reproducibility

• PhytoQuest has grown many the source plants (glasshouse and in field plots) to reduce environment-related variability of the material
• Drying and storage methods are also carefully followed
• Inventory of many plants are kept dried and frozen for resupply of compounds

Phytoquest also focuses on delivering a library product that is easy to use in a range of analytical settings:

Ease of use

• Compounds and fractions are typically supplied at 100µg dried in 96-well micro-titer plates
• Types of plate and plate layout can be defined by the customer

NMR Services for Industry and Academia

¹H, ¹³C, DEPT, 2D, quantitative analyses, structural elucidation.

PhytoQuest Limited offers fast and high quality NMR analysis and interpretation using a Bruker Avance 500 MHz instrument:

• 1D techniques such as ¹H proton and ¹³C DEPT
• 2D correlation (COSY, HMQC, HMBC, NOESY) NMR
• Stereochemical configuration
• Molecular structure determination
• Impurity analysis and identification in samples
• Chemicals, solvents, pharmaceuticals, natural products
• Batch variation/Quality control
• Metabolomics
• Stability studies
• Data Interpreted
• ACD Prediction Software
• Reports and spectra provided in electronic format
• Reasonable rates

For any further information about our services, or in order to receive an estimate, fell free to contact us at following
email address info@phytoquest.co.uk or use the contact form.